Home Programme

Tuesday, 7 December 2004
13:30 - 14:00

The application of trap-TOF technologies to structural analysis of pharmaceutical compounds, proteins and proteoglycans

Roy Edward, Rachel L Martin, Matt Openshaw, Shaun Bilsborough, Chris Sutton, Neil Loftus, Shimadzu Biotech, Manchester UK

It has been recognised over the early years of the proteomics “era” that the high throughput, shotgun-type strategies of the genome projects were not entirely applicable to our understanding of the complex multidimensional nature of the proteome with its isotypes, post-translational modifications and so on.  Despite a determined focus on post-translational modifications, the most common one – glycosylation - has been largely ignored and where structural dissection is essential.  Further, despite the growing interest in protein biomarkers this has been hampered by difficulties to deliver confident identities demanded by clinical scientists and diagnosticians.  Accurate mass determination of product ions is key in the rapid identification of small molecules, such as pharmaceutical drug candidates and metabolites. However, MS/MS analysis is not always sufficient to elucidate the empirical formula of an unknown sample.

This presentation will focus on two core MS technologies that can be applied to proteomics, pharmaceuticals and related areas.  These are based upon a novel trap-TOF configuration combined with dedicated, optimized ESI and MALDI sources and their associated ion introduction systems.  These offer routinely high mass accuracy, consistent resolution and the power of MSn.

Practical applications of the technologies will be described showing the utility and power to elucidate detailed and complex molecular structures.

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