Monday 5 December 2005
9:00- 15:00 at Technopark Zurich
A total of four courses will be offered by in areas directly or indirectly linked to proteomics research:
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Biosafety in Proteomics [CANCELLED]
These courses will be run in parallel sessions so it will not be possible to follow more than one course.
Participation will be limited to 20 persons for each course!
Price: 150 CHF (including coffee break, lunch and handouts)
You may register to one of these Short Courses through the Congress Registration Form (will be open in due time).
Should you require more information about the courses, please contact the Conference Secretariat.
Recombinant antibody phage technology: an introductory course
Prof. Dario Neri, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology Zurich
Scope: Antibody phage technology has revolutionized the way monoclonal antibodies are made, making it possible to isolate binding specificities of choice in just few days, starting from minute amounts of purified antigen and large naive phage-display libraries of antibody fragments. The Course will present the basic experimental strategies for the construction and use of antibody phage libraries. Additionally, issues such as robotization, IPR aspects and pharmaceutical applications of phage-derived antibodies will be discussed.
Audience: The Course is addressed to PhD students and Post-docs wishing to learn how to use antibody phage technology for their research activities in Biology, Chemistry, Pharmaceutical Sciences and/or Medicine. The Course may also be of interest to financial analysts working in the biotech sector.
Benefits: Students will understand the basic principles and experimental techniques associated to antibody phage technology.
Introductory workshop on protein sample preparation and mass spectrometry analysis
Prof. Wilhelm Gruissem, Dr Ralf Schlapbach, Dr Erich Brunner and Dr Sacha Baginsky
Scope: The workshop will provide introductory guidelines to a selection of current sample fractionation and quantitative labeling techniques. Covered aspects include sample fractionation via 2-DE vs. 2D-LC, quantitative labeling approaches (ICAT, iTRAQ, metabolic and enyzmatic), as well as analysis of membrane proteins and post-translational modifications. In addition, primary mass spectrometry analysis of actual samples using different MS systems will be illustrated and discussed.
Audience: The workshop is addressed to PhD students and postdocs aiming to learn the workflows of state-of-the-art proteomics techniques.
Outcome: Attendants will understand the complexity of proteomics methods and technologies and will learn about a selection of powerful tools and approaches to cope with it.
Short course on computational analysis of MS/MS proteomic data sets
Prof. Ruedi Aebersold, Institute for Molecular Systems Biology, Swiss Federal Institute of Technology Zurich
Scope: Proteomic experiment, especially those carried out by LC-MS/MS methods frequently lead to the acquisition of tens to hundreds of thousands of tandem mass spectra. If stable isotope labeling methods are being used, the same datasets also contain accurate quantitative information about the detected proteins. The manual interpretation and validation of these data is no longer possible and it is difficult to consistently interpret the information contained in proteomic profiles without the help of suitable software tools.
In the context of the 2005 SPS proteomic conference we will offer a short course in which the challenges of consistently analyzing LC-MS/MS derived proteomic data are being discussed and a suite of software tools that provide solutions to these challenges will be discussed. The tasks that the software tools perform range from the identification of peptides via sequence database searching of tandem mass spectra, the validation of the search results using statistical principles, the organization of the data in a database to the interpretation of proteomic data in the context of functional modules and biological pathways. The course is based on the tools developed by the Institute for Systems Biology in Seattle (www.proteomecenter.org) under a contract from the National (USA) Heart, Blood and Lung Institute.
Audience: Participant should be very familiar with the structure and manual analysis of peptide MS/MS spectra and should be familiar with Windows based PC’s. It is expected that course participants will be able to load the instructed tools on their computer and to use them for the analysis of LC-MS/MS derived data in their work.
Biosafety in Proteomics [CANCELLED]
Prof. Adriano Aguzzi, Institute of Neuropathology, University of Zurich
